Related Experiment Videos
[Study on Realgar inducing apoptosis in T lymphocytic cell line CEM]
Chen Zhang1, Shi-Lin Huang, Yang Xiang
1TCM Hematology Institute of People's Liberation Army, Dalian, Liaoning Province 116021, China. zcshsmmu@sina.com
Zhong Xi Yi Jie He Xue Bao = Journal of Chinese Integrative Medicine
|September 2, 2004
Abstract
Objective:
To study the Realgar induced T lymphocytic leukemia cell line CEM apoptosis in vitro.
Methods:
CEM cells were incubated with Realgar. Cell proliferation inhibition was determined by MTT. Cell cycle, apoptosis, Apo2.7 and Fas were measured by cytometer.
Results:
Realgar inhibited the proliferation of CEM cell line. The cells treated with Realgar showed a Sub-G(0)/G(1) apoptotic peak in DNA distribution histogram, increment of Apo2.7 protein expression, and arrested cells in G(2)/M phase, but ineffectiveness on Fas expression.
Conclusion:
The Realgar can induce CEM cell apoptosis in vitro.