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Updated: Aug 22, 2026
Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α
Published on: June 14, 2018
[Anti-SARS virus activities of different recombinant human interferons in cell culture system]
Zhao-jun Duan1, Li-lan Zhang, Zhi-ping Xie
1Beijing Jin Di Ke Biotechnology Institute, State Research Center for Biotechnological Engineering, Beijing 100176, China.
Background:
To study the anti-SARS virus activities of different recombinant human interferons on the cell culture system.
Methods:
Anti-SARS virus activities of interferons were determined by using CPE inhibition test in human skeletal muscle sarcoma (Rda) cell culture.
Results:
The average minimum amount of interferon alpha 2b, alpha 1b, beta 1b or omega 1b to inhibit 50% CPE in Rda cell culture was (160.5+/-129.5) IU/ml, (149.0+/-71.7) IU/ml, (69.5+/-61.5) IU/ml, (87.3+/-47.1) IU/ml, respectively or (0.6+/-0.5) ng/ml, (10.6+/-5.1) ng/ml, (3.5+/-3.1) ng/ml, (0.9+/-0.5) ng/ml, respectively.
Conclusion:
All the tested recombinant interferons showed anti-SARS virus activities on the Rda cell culture with different sensitivities.
