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Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells
Published on: July 16, 2013
Fruit extracts antagonize Abeta- or DA-induced deficits in Ca2+ flux in M1-transfected COS-7 cells
James A Joseph1, Derek R Fisher, Amanda N Carey
1USDA-Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111, USA. james.joseph@tufts.edu
Abstract:
Evidence suggests that there is a selective sensitivity to oxidative stress (OSS) among muscarinic receptor (MAChR) subtypes with M1, M2 and M4 showing > OSS than M3 or M5 subtypes in transfected COS-7 cells. This may be important in determining the regional specificity in neuronal aging and Alzheimer disease (AD). We assessed the effectiveness of blueberry (BB) and other high antioxidant (HA) fruit extracts (boysenberry, BY; cranberry, CB; black currant, BC; strawberry, SB; dried plums, DP; and grape, GR) on the toxic effects of Abeta 25-35 (100 microM, 24 hrs) and DA (1 mM, 4 hrs) on calcium buffering (Recovery) following oxotremorine (750 microM) -induced depolarization in M1AChR-transfected COS-7 cells, and on cell viability following DA (4 hrs) exposure. The extracts showed differential levels of Recovery protection in comparisons to the non-supplemented controls that was dependent upon whether DA or Abeta was used as the pretreatment. Interestingly, assessments of DA-induced decrements in viability revealed that all of the extracts had some protective effects. These findings suggest that the putative toxic effects of Abeta or DA might be reduced by HA fruit extracts.
Insights
High antioxidant fruit extracts, like blueberry, show protective effects against oxidative stress in muscarinic receptor cells. These findings suggest potential benefits for neuronal aging and Alzheimer's disease.
Area of Science:
- Neuroscience
- Biochemistry
- Pharmacology
Background:
- Muscarinic acetylcholine receptor (MAChR) subtypes exhibit selective sensitivity to oxidative stress (OSS).
- M1, M2, and M4 MAChR subtypes show greater OSS than M3 or M5 subtypes.
- This differential sensitivity may influence neuronal aging and Alzheimer's disease (AD) pathology.
Purpose of the Study:
- To evaluate the protective effects of high antioxidant (HA) fruit extracts against oxidative damage in M1AChR-transfected cells.
- To assess the impact of blueberry and other fruit extracts on cellular recovery and viability under toxic conditions.
Main Methods:
- M1AChR-transfected COS-7 cells were exposed to amyloid-beta (Abeta) 25-35 or dopamine (DA) to induce toxicity.
- The effectiveness of blueberry and other HA fruit extracts was measured by their ability to restore calcium buffering (Recovery) after oxotremorine-induced depolarization.
- Cell viability was assessed following DA exposure.
Main Results:
- HA fruit extracts demonstrated varying levels of protection for calcium buffering, depending on whether Abeta or DA was used for pretreatment.
- All tested HA fruit extracts provided some protection against DA-induced decrements in cell viability.
- Blueberry and other fruit extracts may mitigate the toxic effects of Abeta and DA on neuronal cells.
Conclusions:
- High antioxidant fruit extracts show promise in protecting against oxidative stress-induced neuronal damage.
- These findings suggest a potential therapeutic role for HA fruit extracts in conditions like Alzheimer's disease.
- Further research into the specific compounds and mechanisms of action is warranted.
