Primary bone-derived cell colonization of unconditioned and pre-conditioned Bioglass 45S5 surfaces in vitro

Samples of Bioglass 45S5, a bioactive glass that reacts chemically on immersion in an aqueous environment and following implantation can bond with bone, were immersed in culture medium with foetal calf serum as a source of proteins for periods of 1-11 days. Energy dispersive spectroscopy (EDS) revealed that over 11 days immersion, the Bioglass surface was rich in Si relative to Ca and P and scanning electron microscopy (SEM) showed that the Bioglass developed surface reaction layers. Samples conditioned in culture medium and previously untreated samples were used as substrates for primary osteoblast or periosteal cell cultures, to allow comparison of the effects of different Bioglass surface development, on subsequent cell attachment. SEM examination revealed that pre-conditioned Bioglass was a more suitable substrate for osteoblast colonization than previously undeveloped Bioglass, whereas periosteal cells colonized all samples equally well and more rapidly than osteoblasts.