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Coactivators p300 and PCAF physically and functionally interact with the foamy viral trans-activator.
Helmut Bannert1, Walter Muranyi, Vasily V Ogryzko
1Department of Retroviral Gene Expression, German Cancer Research Center, Applied Tumor Virology, Heidelberg, Germany. h.bannert@dkfz-heidelberg.de
BMC Molecular Biology
|September 8, 2004
Summary
This study identifies p300 and PCAF as key coactivators that directly interact with the foamy virus Bel1/Tas trans-activator, enhancing gene expression. These findings reveal novel cellular partners for retroviral trans-activation mechanisms.
Area of Science:
- Retroviral gene regulation
- Molecular biology
- Virology
Background:
- Foamy virus Bel1/Tas trans-activators regulate gene expression by binding to Bel1 response elements (BRE).
- Cellular coactivators interacting with Bel1/Tas were previously unknown.
Purpose of the Study:
- To identify and characterize cellular coactivators that interact with the foamy virus Bel1/Tas trans-activator.
- To elucidate the role of these coactivators in Bel1/Tas-mediated transcriptional trans-activation.
Main Methods:
- Transient expression assays
- Co-immunoprecipitation experiments
- Pull-down assays
- Western blot analysis
Main Results:
- p300 and histone acetyltransferase PCAF were identified as coactivators that specifically interact with Bel1/Tas in vivo.
- Bel1/Tas-mediated trans-activation was enhanced by p300, PCAF, and SRC-1.
- The Bel1/Tas-interacting region was mapped to the C/H1 domain of p300.
- SRC-1 enhanced trans-activation without direct interaction with Bel1 protein.
Conclusions:
- p300 and PCAF are functional partners that directly interact with Bel1/Tas.
- A C/H1 expression plasmid partially inhibited Bel1/Tas-mediated activation of p300.
- This study provides the first report of a C/H1 domain-interacting retroviral trans-activator.