Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Single-cell microbiology: tools, technologies, and applications.

Byron F Brehm-Stecher1, Eric A Johnson

  • 1Department of Food Microbiology and Toxicology, University of Wisconsin-Madison Food Research Institute, 1925 Willow Drive, Madison, WI 53706, USA.

Microbiology and Molecular Biology Reviews : MMBR
|September 9, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Manufacture of the first FDA-approved batches of 900 kilodalton crystalline type a botulinum toxin for clinical use: a personal remembrance.

Journal of neural transmission (Vienna, Austria : 1996)·2026
Same author

Enteric botulinum neurotoxins facilitate infection by <i>Salmonella</i> and <i>Shigella</i>.

Science advances·2026
Same author

Korean botulinum toxins.

Journal of neural transmission (Vienna, Austria : 1996)·2026
Same author

Iatrogenic botulism: a risk for botulinum toxin's medical use?

Journal of neural transmission (Vienna, Austria : 1996)·2026
Same author

Single-Tube Capture, Concentration, and Genomic Extraction of a Human Norovirus Surrogate Using Magnetic Ionic Liquids.

Analytical chemistry·2025
Same author

A Data-driven Age-related Macular Degeneration Severity Scoring System Leveraging the AREDS Studies and Clinical Electronic Medical Records.

Ophthalmology·2025
Same journal

Structure, assembly, and mechanism of the MmpL family of transporters.

Microbiology and molecular biology reviews : MMBR·2026
Same journal

Killer meiotic drivers in fungi.

Microbiology and molecular biology reviews : MMBR·2026
Same journal

From dividing to dormant: embracing the full activity spectrum for environmental microorganisms.

Microbiology and molecular biology reviews : MMBR·2026
Same journal

Role of TMDs in Class I viral fusion proteins.

Microbiology and molecular biology reviews : MMBR·2026
Same journal

The microbiome in reptile health, disease, and ecology.

Microbiology and molecular biology reviews : MMBR·2026
Same journal

Overcoming the energy-dependent barrier to aminoglycoside uptake: multimodal strategies to sensitize <i>Staphylococcus aureus</i> persisters.

Microbiology and molecular biology reviews : MMBR·2026
See all related articles

Microbiology studies are shifting from population-level data to single-cell analysis. This approach reveals crucial cellular heterogeneity, impacting antibiotic resistance and disease potential.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biotechnology

Background:

  • Traditional microbiology relies on population-level data, inferring cellular behavior.
  • This averaging effect masks significant genetic and phenotypic heterogeneity among individual microorganisms.
  • Such heterogeneity impacts antibiotic resistance, industrial processes, and pathogen virulence.

Purpose of the Study:

  • To highlight the limitations of population-level microbiology.
  • To emphasize the importance of studying cellular heterogeneity at the single-cell level.
  • To discuss the impact of technological advancements on single-cell microbiology.

Main Methods:

  • Review of traditional population-level microbiological studies.
  • Analysis of technological advancements enabling single-cell observation.

Related Experiment Videos

  • Comparison of population-level versus single-cell data interpretation.

    • Main Results:

    • Population-level studies provide averaged data, obscuring individual cell variations.
    • Single-cell analysis overcomes averaging effects, revealing discrete microbiological phenomena.
    • New technologies facilitate unprecedented detail in characterizing microbial activities and interactions.

    Conclusions:

    • Studying microbial heterogeneity at the single-cell level is crucial.
    • Single-cell approaches offer unique insights unavailable through traditional methods.
    • Technological progress is revolutionizing the study of individual microbial cells and their functions.