[Semen analysis of DNA fluorescence detection and two other routine analyzing methods]

Area of Science:

• Reproductive biology
• Andrology
• Medical diagnostics

Context:

• Semen analysis is crucial for evaluating male infertility.
• Traditional methods like microscopic analysis and grey-scale CASA have limitations in distinguishing sperm from debris and assessing viability.
• Accurate sperm density measurement is vital for fertility assessments.

Purpose:

• To compare the accuracy and reliability of a novel computer-aided sperm analysis (CASA) system utilizing DNA fluorescence (fluorescence CASA) against conventional semen analysis techniques.
• To evaluate the system's ability to differentiate sperm from non-sperm granules and living from dead sperm.
• To assess the concordance of sperm density measurements obtained via fluorescence CASA with those from microscopic analysis and grey-scale CASA.

Summary:

• Fluorescence CASA demonstrated superior performance in distinguishing sperm from non-sperm granules and differentiating between live and dead sperm.
• Significant disparities were observed in sperm density measurements between fluorescence CASA and both microscopic analysis (49.06% +/- 49.87%) and grey-scale CASA (43.39% +/- 25.56%).
• The fluorescence CASA method showed higher accuracy and better adherence to the latest WHO laboratory manual standards for semen examination.

Impact:

• The findings suggest that fluorescence CASA is a valuable and recommended tool for clinical semen analysis, particularly for precise sperm density determination.
• This advanced method has the potential to improve the diagnostic accuracy in male infertility evaluations.
• Adoption of fluorescence CASA could lead to more reliable and standardized semen testing protocols.