Micro parallel liquid chromatography: enabling technology for discovery analytical chemistry.

Area of Science:

• Analytical Chemistry
• Chromatography
• Drug Discovery

Background:

• Compound libraries have expanded significantly due to combinatorial chemistry, increasing the demand for high-throughput analysis.
• Analytical chemistry faces bottlenecks in research and development productivity, necessitating advancements in instrumentation.
• New technologies for lead compound generation require enhanced analytical capabilities.

Purpose of the Study:

• To introduce the Veloce micro parallel liquid chromatography system.
• To demonstrate the system's capability for high-throughput analysis of compound libraries.
• To present applications in compound purity screening, log P determination, and aqueous solubility assessment.

Main Methods:

• Utilized the Nanostream Veloce micro parallel liquid chromatography system.
• Analyzed 24 samples in parallel with minimal sample volume (0.5 microl).
• Applied the system to various discovery analytical applications.

Main Results:

• The Veloce system enables parallel analysis of 24 samples, significantly increasing throughput.
• Demonstrated scalability for diverse analytical needs, from purity screening to property determination.
• Representative data showcase the system's effectiveness in discovery analytical applications.

Conclusions:

• The Veloce system addresses the need for increased analytical throughput in drug discovery.
• It offers a scalable solution for bottlenecks in compound library screening and property assessment.
• This technology supports enhanced research and development productivity in analytical chemistry.