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Updated: Aug 22, 2026
Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
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Development of a real-time PCR assay using SYBR Green I for provirus load quantification in a murine model of AIDS
Anna Casabianca1, Chiara Orlandi, Alessandra Fraternale
1Institute di Biological Chemistry Giorgio Fornaini, University of Urbino, Italy.
Abstract:
A real-time PCR assay using SYBR Green I for quantification of provirus load in a murine model of AIDS (i.e., LP-BM5 infection) was developed and validated. In this method, data are normalized against the 18S rRNA gene. The method has a dynamic range of 8 logs and a sensitivity of one copy.
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