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Related Experiment Videos

Epidermal label-retaining cells: background and recent applications.

Kristin M Braun1, Fiona M Watt

  • 1Keratinocyte Laboratory, Cancer Research UK London Research Institute, London, UK.

The Journal of Investigative Dermatology. Symposium Proceedings
|September 17, 2004
PubMed
Summary

Label-retaining cells (LRC) in mouse epidermis, identified via thymidine or bromo-deoxyuridine injections, are key indicators of epidermal stem cells. Recent methods allow visualization and purification of these cells for stem cell marker screening.

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Area of Science:

  • Dermatology
  • Cell Biology
  • Stem Cell Research

Background:

  • Epidermal label-retaining cells (LRC) are identified by persistent labeling after neonatal injections of 3H-thymidine or 5-bromo-2'-deoxyuridine.
  • Label retention, while a marker of proliferative history, is increasingly recognized as a characteristic of epidermal stem cells.

Purpose of the Study:

  • To review the early literature on epidermal label-retaining cells (LRC).
  • To highlight recent applications of LRC identification and purification.
  • To explore the utility of LRC in identifying epidermal stem cell markers.

Main Methods:

  • Neonatal mice received repeated injections of 3H-thymidine or 5-bromo-2'-deoxyuridine.
  • Label retention was assessed in adult mice.

Related Experiment Videos

  • Whole-mount labeling techniques were employed for epidermal visualization.
  • Purified LRC were utilized for stem cell marker screening.

    • Main Results:

    • Label retention serves as a reliable marker for identifying cells with a specific proliferative history in the epidermis.
    • Recent advancements enable effective whole-mount visualization of LRC in the epidermis.
    • Purified LRC can be used to discover and validate markers for the epidermal stem cell compartment.

    Conclusions:

    • LRC are strong candidates for epidermal stem cells.
    • Novel techniques enhance the study of LRC and epidermal stem cell biology.
    • LRC purification offers a powerful approach for identifying novel epidermal stem cell markers.