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Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
Published on: April 12, 2019
Base-dependent competitive adsorption of single-stranded DNA on gold
Hiromi Kimura-Suda1, Dmitri Y Petrovykh, Michael J Tarlov
1National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.
Journal of the American Chemical Society
|September 17, 2004
Summary
DNA oligonucleotides show a strong base-dependent adsorption to gold surfaces. Adenine-rich DNA dominates adsorption, even displacing thiolated DNA and denaturing DNA duplexes.
Area of Science:
- Surface science
- Biomaterials science
- Nanotechnology
Background:
- Understanding DNA adsorption on gold is crucial for biosensor development.
- Oligonucleotide properties influence their interaction with surfaces.
- Gold surfaces are widely used in bio-interfacing applications.
Purpose of the Study:
- To characterize the adsorption behavior of single-stranded DNA homo-oligonucleotides on gold.
- To investigate the competitive adsorption dynamics between different DNA sequences.
- To determine the base-specific affinity of DNA adsorption on gold surfaces.
Main Methods:
- Room-temperature adsorption experiments using polycrystalline gold films.
- Analysis of adsorbed DNA using Fourier Transform Infrared (FTIR) spectroscopy.
- Surface characterization via X-ray Photoelectron Spectroscopy (XPS).
Main Results:
- Oligonucleotides exhibit a base-dependent adsorption affinity: Adenine (A) > Cytosine (C) >= Guanine (G) > Thymine (T).
- Oligo(dA) demonstrates dominant adsorption, outcompeting other unmodified oligonucleotides.
- Oligo(dA) effectively competes with thiolated oligonucleotides and can denature DNA duplexes on gold.
Conclusions:
- The adsorption of DNA on gold is strongly influenced by nucleotide base composition.
- Adenine-rich DNA sequences possess a significantly higher affinity for gold surfaces.
- These findings have implications for designing DNA-based nanostructures and biosensors.
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