Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microarray TRAP--a high-throughput assay to quantitate telomerase activity.

Katarzyna Heller-Uszynska1, Andrzej Kilian

  • 1CAMBIA: Center for the Application of Molecular Biology to International Agriculture, G.P.O. Box 3200, Canberra, ACT 2601, Australia.

Biochemical and Biophysical Research Communications
|September 17, 2004
PubMed
Summary

A new microarray-based assay (MTRAP) offers reliable, high-throughput quantification of telomerase activity, overcoming limitations of the traditional TRAP assay for telomere research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

BrAPI v2: real-world applications for data integration and collaboration in the breeding and genetics community.

Database : the journal of biological databases and curation·2025
Same author

Genome-wide association study of seedling leaf rust resistance in European winter wheat cultivars.

Journal of applied genetics·2025
Same author

Erratum to: Targeted genotyping-by-sequencing of potato and data analysis with R/polyBreedR.

The plant genome·2025
Same author

Estimating Demographic Parameters for Bearded Seals, <i>Erignathus barbatus</i>, in Alaska Using Close-Kin Mark-Recapture Methods.

Evolutionary applications·2024
Same author

Targeted genotyping-by-sequencing of potato and data analysis with R/polyBreedR.

The plant genome·2024
Same author

Correction: Identification of quantitative trait loci associated with leaf rust resistance in rye by precision mapping.

BMC plant biology·2024

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • Telomerase plays a crucial role in telomere maintenance, cell proliferation, tumor development, and cell immortalization.
  • The conventional Telomeric Repeat Amplification Protocol (TRAP) assay, while sensitive, has limitations including lack of quantification, product size variability, and low throughput.
  • These limitations hinder comprehensive studies on telomerase's role in biological processes.

Purpose of the Study:

  • To develop a novel assay for reliable and high-throughput quantification of telomerase activity.
  • To overcome the limitations of the existing TRAP assay, particularly in quantification and throughput.
  • To provide a robust tool for massive quantitation of telomerase activity in biological research.

Main Methods:

Related Experiment Videos

  • Development and optimization of a microarray-based TRAP (MTRAP) assay using rice cell suspension telomerase extract.
  • Integration of microarray technology with a modified TRAP assay to enhance specificity and reliability.
  • Incorporation of built-in controls for product integrity and false negatives to ensure accurate results.

Main Results:

  • The MTRAP assay enables specific, reliable, and linear quantification of telomerase activity.
  • Achieved sensitivity comparable to radioisotope-based TRAP assays.
  • Demonstrated high-throughput capability with built-in quality control mechanisms.

Conclusions:

  • The MTRAP assay is a significant advancement over the traditional TRAP assay.
  • It provides a reliable and efficient tool for large-scale quantitation of telomerase activity.
  • This new assay will facilitate deeper understanding of telomerase function in various biological contexts, including cancer research.