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Updated: Aug 22, 2026

Electrophoretic Separation of Proteins
Published on: June 12, 2008
Measuring the electrophoretic mobility of concentrated suspensions in nonaqueous media
Alberto T Pérez1, Elisabeth Lemaire
1Laboratoire de Physique de la Matière Condensée, CNRS Université de Nice, UMR 6622, Parc Valrose, 06108 Nice cedex 2, France. alberto@us.es
Abstract:
We present a new method of measuring the electrophoretic mobility of a particle in a concentrated suspension. The method is used to measure the electrophoretic mobility of PMMA particles (diameter 10 microm) suspended in a mixture of liquid hydrocarbons. The particle volume fraction of the suspension is varied from 0 up to 0.30 and the resulting variation of the electrophoretic mobility is discussed. The suspending liquid is such that its refractive index is very close to that of the particles. Thus the suspension is almost transparent and it is possible to follow through a microscope the motion of one particle. The suspension is subjected to a low-frequency electric field (0.5 Hz). The cell containing the suspension is mounted on a piezoelectric crystal. The displacement that compensates for the particle motion (when the particle image is steady) is determined.
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