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Related Experiment Videos

Methicillin-resistant staphylococci isolated from animals.

E van Duijkeren1, A T A Box, M E O C Heck

  • 1Bacteriology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, P.O. Box 80165, 3508 TD Utrecht, The Netherlands. E.duijkeren@vet.uu.nl

Veterinary Microbiology
|September 24, 2004
PubMed
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Methicillin resistance in animal staphylococci was investigated. Some strains carried the mecA gene, and one MRSA strain showed homology to human strains, suggesting potential human-to-dog transmission.

Area of Science:

  • Veterinary Microbiology
  • Antimicrobial Resistance
  • Molecular Biology

Background:

  • Staphylococci are common bacteria found in animals.
  • Methicillin resistance in staphylococci is a growing public health concern.
  • Understanding the prevalence and mechanisms of methicillin resistance in animal isolates is crucial.

Purpose of the Study:

  • To screen staphylococci isolated from animals for methicillin resistance.
  • To identify the presence of the mecA gene in resistant strains.
  • To investigate the genetic relatedness of methicillin-resistant staphylococci (MRS) from animals.

Main Methods:

  • Oxacillin agar screening for methicillin resistance.
  • Polymerase chain reaction (PCR) to detect the mecA gene.

Related Experiment Videos

  • 16S rDNA analysis for isolate identification.
  • Agar diffusion method for antimicrobial susceptibility testing.
  • Pulsed-field gel electrophoresis (PFGE) for genetic profiling.

    • Main Results:

    • Six out of 11 multidrug-resistant staphylococci were mecA-positive, including two methicillin-resistant Staphylococcus aureus (MRSA) and four Staphylococcus haemolyticus.
    • Four out of five oxacillin-resistant coagulase-negative staphylococci (CNS) screened were mecA-positive.
    • PFGE revealed distinct patterns for most animal-derived mecA-positive staphylococci.
    • One MRSA isolate from a dog exhibited homology to a human epidemic MRSA cluster.

    Conclusions:

    • Methicillin resistance is present in staphylococci isolated from animals.
    • The mecA gene is a key marker for methicillin resistance in these isolates.
    • Evidence suggests potential transmission of MRSA between humans and dogs.