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Nanosecond pulsed electric fields modulate cell function through intracellular signal transduction mechanisms
Stephen J Beebe1, Peter F Blackmore, Jody White
1Center for Pediatric Research, Eastern Virginia Medical School, Children's Hospital for The King's Daughters, Norfolk, VA, USA. sbeebe@chkd.com
Physiological Measurement
|September 24, 2004
Summary
Nanosecond pulsed electric fields (nsPEF) modulate cell function by affecting intracellular signaling and plasma membrane interactions. These findings offer new tools for studying cell mechanisms and potential cancer and gene therapies.
Area of Science:
- Cellular biology
- Biophysics
- Electroporation
Background:
- Nanosecond pulsed electric fields (nsPEF) are a novel tool for cell manipulation.
- Understanding nsPEF effects on cell structure and function is crucial for their application.
Purpose of the Study:
- To investigate the impact of nsPEF on mammalian cell structure and function.
- To explore the distinct mechanisms of nsPEF-induced plasma membrane (PM) modulation.
- To assess the potential of nsPEF in cancer and gene therapy.
Main Methods:
- Exposure of mammalian cells (HL-60, Jurkat) and mouse fibrosarcoma tumors to nsPEF with varying parameters.
- Analysis of plasma membrane (PM) effects, intracellular calcium signaling, and apoptosis induction.
- Evaluation of gene expression (green fluorescent protein) following nsPEF treatment.
Main Results:
- Decreasing nsPEF pulse durations shift effects from PM to intracellular signaling.
- nsPEF induce unique PM modulations distinct from classical electroporation.
- nsPEF trigger apoptosis in mammalian cells and tumors, and enhance gene expression.
Conclusions:
- nsPEF activate intracellular mechanisms influencing cell fate and function.
- nsPEF represent a valuable tool for probing signal transduction pathways.
- Potential therapeutic applications for nsPEF in cancer and gene therapy are indicated.