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Average protein density is a molecular-weight-dependent function.

Hannes Fischer1, Igor Polikarpov, Aldo F Craievich

  • 1Institute of Physics, University of São Paulo, Rua do Matao Travessa R, 187 CEP 05508-900, São Paulo-SP, Brazil.

Protein Science : a Publication of the Protein Society
|September 25, 2004
PubMed
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The average protein density is not constant and varies with molecular weight. Smaller proteins (<20 kDa) show increased density, prompting a new estimation method based on molecular weight.

Area of Science:

  • Biophysics
  • Structural Biology

Background:

  • Protein mass density is a fundamental biophysical property.
  • It is crucial for techniques like X-ray crystallography and analytical ultracentrifugation.
  • Accurate density values are needed for protein structure and oligomer studies.

Purpose of the Study:

  • To critically analyze existing data on protein mass density.
  • To determine if protein density is a constant value.
  • To develop a more accurate method for estimating protein density.

Main Methods:

  • Comprehensive analysis of published literature.
  • Review of theoretical investigations.
  • Evaluation of experimental data.

Main Results:

Related Experiment Videos

  • Protein mass density is not a constant value.
  • Proteins with molecular weights below 20 kDa show a positive deviation in density.
  • This deviation increases as molecular weight decreases.

Conclusions:

  • The assumption of constant protein density is inaccurate.
  • A novel, simple function correlating molecular weight and density is proposed.
  • This function offers improved accuracy for estimating average protein density.