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Updated: Aug 22, 2026

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Biosynthetic pathway for the C45 polyprenol, solanesol, in tobacco
Ei'ichiro Fukusaki1, Shinya Takeno, Takeshi Bamba
1Department of Biotechnology, Graduate School of Engineering, Osaka University, Yamadaoka, Suita, Japan. fukusaki@bio.eng.osaka-u.ac.jp
Abstract:
Feeding experiments were independently performed with [1-13C]deoxy-D-xylulose triacetate and (RS)-[2-13C]mevalonolactone in the tobacco plant. The labeling pattern for solanesol was elucidated to reveal that the isoprene moiety of solanesol would be derived from deoxy-xylulose. The result strongly suggests that tobacco solanesol is biosynthesized via the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway.
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