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Systemic and regional acetate kinetics in dogs
B Bleiberg1, T R Beers, M Persson
1Endocrine Research Unit, Mayo Medical School, Rochester, Minnesota 55905.
The American Journal of Physiology
|February 1, 1992
Summary
Dogs produce and use acetate, a key energy source, in various organs like the intestine and liver. This acetate metabolism is crucial for energy transport, especially during fasting.
Area of Science:
- Physiology
- Metabolic Biochemistry
Background:
- Acetate metabolism in non-ruminant species remains poorly understood.
- Key sites of acetate production and uptake are not well-defined.
Purpose of the Study:
- To investigate the systemic and regional production and uptake of acetate in conscious dogs.
- To quantify acetate turnover, oxidation, and clearance in a non-ruminant model.
Main Methods:
- Utilized radiolabeled acetate ([1-14C]acetate) infusion in mongrel dogs (n=11).
- Collected blood samples from multiple arterial and venous sites (femoral, portal, hepatic, renal).
- Measured 14CO2 excretion, plasma acetate concentration, and specific activity to determine regional acetate exchange.
Main Results:
- Systemic acetate turnover was 8.8 ± 1.9 µmol·kg−1·min−1, contributing ~5% to energy expenditure.
- Simultaneous acetate uptake and release occurred in the intestine, liver, kidney, and hindlimb.
- The intestine was the primary acetate producer, while the liver was the main site of uptake.
- Acetate oxidation accounted for 77 ± 4% of systemic turnover.
- Systemic clearance and tissue fractional extraction of acetate were significantly higher than for glucose, fatty acids, lactate, or amino acids.
Conclusions:
- Most tissues in dogs exhibit simultaneous acetate uptake and release.
- This bidirectional acetate flux may serve as a mechanism for interorgan energy transport.
- Acetate plays a significant role in energy metabolism, particularly during caloric deprivation.