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Isolation and characterization of a bradykinin-potentiating peptide from a bovine peptic hemoglobin hydrolysate
J M Piot1, Q Zhao, D Guillochon
1Laboratoire de Technologie des Substances Naturelles, BP 179-IUT A Lille I, Villeneuve d'Ascq, France.
FEBS Letters
|March 24, 1992
Abstract:
A bradykinin potentiating peptide was isolated from a peptic bovine hemoglobin hydrolysate, by the use of reversed-phase high-performance liquid chromatography (RP-HPLC). Its primary structure, determined by fast atom bombardment (FAB) and tandem mass spectrometry (MS/MS), was identical to fragment 129-134 of the alpha-chain of bovine hemoglobin. The bradykinin potency of this peptide, as exhibited by the guinea-pig ileum contraction, was significant and comparable with some others previously described.