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Bovine hydroxyindole-O-methyltransferase. Significant sequence revision.
S J Donohue1, P H Roseboom, D C Klein
1Section on Neuroendocrinology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892.
The Journal of Biological Chemistry
|March 15, 1992
Summary
Revised bovine hydroxyindole-O-methyltransferase (HIOMT) gene sequencing reveals significant differences. These corrections impact 28% of the protein sequence, refining future melatonin synthesis research.
Area of Science:
- Biochemistry
- Molecular Biology
- Genetics
Background:
- Hydroxyindole-O-methyltransferase (HIOMT) is crucial for melatonin synthesis.
- Previous human and bovine HIOMT sequence comparisons showed discrepancies.
- These differences hindered accurate alignment and homology assessment.
Purpose of the Study:
- To re-evaluate and correct the bovine HIOMT nucleotide and amino acid sequences.
- To resolve discrepancies between human and bovine HIOMT sequences.
- To provide an accurate sequence for future HIOMT research.
Main Methods:
- Re-sequencing of the original bovine HIOMT cDNA clone.
- Comparative analysis of the revised bovine sequence with the previously published sequence.
- Analysis of predicted post-translational modifications based on the revised amino acid sequence.
Main Results:
- The revised bovine HIOMT nucleotide sequence contains 23 differences from the published version.
- The deduced amino acid sequence is significantly altered in two regions (96 residues, 28% of the protein).
- The corrected sequence predicts different post-translational modifications.
Conclusions:
- The re-sequencing provides a more accurate bovine HIOMT sequence.
- This accurate sequence is essential for reliable future studies on HIOMT.
- Improved primers, antisera, and probes can be designed for HIOMT research.