Ventilatory effects of gap junction blockade in the NTS in awake rats

We tested the hypothesis that focally perfusing carbenoxolone, which blocks gap junctions, into the nucleus tractus solitarius (NTS) would reduce the ventilatory response to CO(2). We measured minute ventilation (V(E)), tidal volume (V(T)) and respiratory frequency (F(R)) responses to increasing concentrations of inspired CO(2) (F(I)(CO(2) = 0-8%) in rats during wakefulness. Focal perfusion of acetazolamide (10 microM) into the NTS increased V(E) and V(T) during exposure to room air. Carbenoxolone (300 microM) decreased the V(E) and V(T) response to CO(2) when perfused within, but not adjacent to the NTS in animals less than 10 weeks of age. F(R) was decreased at F(I)(CO(2) = 4% in these animals. Carbenoxolone did not decrease V(E), V(T) or F(R) in animals 10 weeks of age and older. Carbenoxolone did not decrease V(E), V(T) or F(R) when focally perfused outside the NTS at any age tested. The NTS is an important CO(2) chemosensory site at all ages, and gap junctions amplify the ventilatory response to CO(2) in animals less than 10 weeks of age.