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Related Experiment Videos

SpeedScreen: The "missing link" between genomics and lead discovery.

Hartmut Zehender1, Francois Le Goff, Natalie Lehmann

  • 1Novartis Institutes for Biomedical Research, Discovery Technologies/Basel Screening Operations, 4002 Basel, Switzerland. hartmut.zehender@pharma.novartis.com

Journal of Biomolecular Screening
|September 29, 2004
PubMed
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SpeedScreen is a new label-free, in-solution method for high-throughput screening (HTS). This affinity-based selection technology, combined with mass spectrometry, offers an efficient approach for drug discovery campaigns.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Drug Discovery

Background:

  • High-throughput screening (HTS) is crucial for identifying drug candidates.
  • Existing HTS methods often require labels or complex assay development.
  • There is a need for efficient, label-free screening technologies.

Purpose of the Study:

  • To introduce SpeedScreen, a novel label-free, in-solution affinity selection methodology for HTS.
  • To describe the principles of assay development, HTS, and data analysis using SpeedScreen.
  • To discuss the advantages and limitations of SpeedScreen compared to other screening technologies.

Main Methods:

  • SpeedScreen utilizes in-solution affinity selection.
  • The protocol integrates size exclusion chromatography (SEC).

Related Experiment Videos

  • Microbore-liquid-chromatography/electrospray-ionization mass spectrometry (micro-LC/ESI-MS) is employed for detection.

    • Main Results:

    • SpeedScreen provides a label-free, in-solution approach for HTS.
    • The technology enables efficient affinity selection and analysis.
    • An example campaign demonstrates the application of SpeedScreen in HTS.

    Conclusions:

    • SpeedScreen is an innovative affinity selection concept for HTS.
    • The methodology offers advantages over alternative screening technologies.
    • This approach facilitates efficient drug discovery campaigns.