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Relationship between bacterial infection and evaluation using a laser fluorescence device, DIAGNOdent
Yukiteru Iwami1, Ayako Shimizu, Masahiro Narimatsu
1Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, Osaka, Japan. y-iwami@dent.osaka-u.ac.jp
European Journal of Oral Sciences
|October 2, 2004
Summary
This study found a strong correlation between laser fluorescence device (DIAGNOdent) readings and bacterial presence in carious dentin, as confirmed by polymerase chain reaction (PCR). Higher DIAGNOdent values indicate a greater likelihood of bacterial detection in dentin caries.
Area of Science:
- Dentistry
- Microbiology
- Diagnostic Technology
Background:
- Bacterial infections are a primary cause of dentin caries.
- Accurate detection of bacterial presence in carious dentin is crucial for effective treatment.
- Existing diagnostic methods require validation for bacterial detection.
Purpose of the Study:
- To investigate the relationship between bacterial detection in carious dentin using polymerase chain reaction (PCR) and laser fluorescence (DIAGNOdent).
- To establish the diagnostic accuracy of DIAGNOdent for identifying bacterial presence in dentin caries.
Main Methods:
- An in vitro study using 10 extracted molars with occlusal dentin caries and 3 sound molars.
- Dentin samples were progressively removed towards the pulp chamber.
- Bacterial DNA was detected using PCR (16S rDNA primers), and dentin surfaces were evaluated with DIAGNOdent.
Main Results:
- Bacterial detection rates increased proportionally with higher DIAGNOdent values.
- No bacteria were detected at DIAGNOdent values below 15.6.
- The area under the receiver operating characteristic (ROC) curve was 0.91, indicating high diagnostic accuracy for DIAGNOdent.
Conclusions:
- DIAGNOdent readings show a significant correlation with the presence of bacteria in carious dentin.
- DIAGNOdent is a reliable tool for assessing bacterial load in dentin caries.
- This finding supports the use of DIAGNOdent in clinical settings for caries management.