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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Updated: Mar 1, 2026

A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces

A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces

Published on: March 1, 2017

In control: systematic assessment of microarray performance.

Harm van Bakel¹, Frank C P Holstege

¹University Medical Center Utrecht, PO Box 85060, 3508 AB Utrecht, The Netherlands.

|October 2, 2004

Summary

This summary is machine-generated.

Reliability of DNA microarray measurements is crucial for life sciences. This review advocates for external RNA controls as the most versatile standard for assessing microarray performance, accuracy, and precision.

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DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

Area of Science:

Life Sciences
Molecular Biology
Genomics

Background:

DNA microarrays are widely used for expression profiling in life sciences.
Assessing the reliability of microarray data is challenging due to experimental complexity and diverse platforms.
There is a growing demand for standardized methods in microarray analysis.

Purpose of the Study:

To review the challenges in assessing microarray performance.
To highlight the importance of accuracy and precision in microarray measurements.
To propose external RNA controls as a versatile standard for performance evaluation.

Main Methods:

Review of existing approaches for microarray performance assessment.
Argument for the adoption of external RNA controls as a standardized system.
Discussion on the implementation of external controls and related factors.

Main Results:

External RNA controls are identified as the most versatile system for determining microarray performance.
The review outlines how such standards could be implemented.
Importance of probe sequence availability and quantification of labelled material are discussed.

Conclusions:

Standardized assessment of microarray performance is essential for reliable life science research.
External RNA controls offer a robust and versatile solution for evaluating microarray accuracy and precision.
Implementation of external controls alongside attention to probe details will enhance data quality.