Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Murine Asb-17 expression during mouse testis development and spermatogenesis.

Kye-Seong Kim1, Myung-Sun Kim, Soo-Kyung Kim

  • 1Cell and Gene Therapy Research Institute, Graduate School of Life Science and Biotechnology, Pochon CHA University, CHA General Hospital, Seoul, Korea.

Zygote (Cambridge, England)
|October 6, 2004
PubMed
Summary

Researchers identified a mouse gene, mAsb-17, crucial for male reproductive health. This gene is specifically expressed in the testes, playing a key role in sperm development and overall testicular function.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spatially resolved quantification of wheat kernel vitreousness using hyperspectral imaging and spectral unmixing.

Frontiers in plant science·2026
Same author

The ubiquitin-proteasome system: A master regulator of cell cycle progression.

Pharmacological research·2026
Same author

The USP19-DnaJC7 Axis Stabilizes p53 in Cisplatin-Treated Epithelial Ovarian Cancer.

Cells·2026
Same author

Key regulatory pathways in recurrent pregnancy loss: Integrating trophoblast dysfunction, angiogenesis, and inflammatory signaling.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

Deubiquitinating enzymes in cervical cancer: Molecular mechanisms and therapeutic implications (Review).

International journal of oncology·2026
Same author

cP1P Maintains Long-Term Pluripotency in Human Pluripotent Stem Cells.

International journal of stem cells·2026

Area of Science:

  • Molecular Biology
  • Reproductive Biology
  • Genetics

Background:

  • The Asb (Ankyrin Repeat and SOCS Box) gene family encodes proteins with diverse biological functions.
  • Understanding the specific roles of individual Asb family members is essential for elucidating complex biological processes.

Purpose of the Study:

  • To isolate and characterize a novel murine Asb gene, designated mAsb-17.
  • To investigate the expression pattern and potential function of mAsb-17 in the mouse testis.

Main Methods:

  • Isolation of mAsb-17 using Reverse Transcription Polymerase Chain Reaction (RT-PCR) with primers from GenBank.
  • Sequence analysis to determine the polypeptide characteristics and identify conserved domains (ankyrin repeats, SOCS box).
  • Expression analysis using Northern blot and RT-PCR to determine tissue specificity and developmental expression patterns.

Related Experiment Videos

Main Results:

  • mAsb-17 encodes a 295 amino acid protein (approx. 34 kDa) with two ankyrin repeats and one SOCS box.
  • High sequence identity of mASB-17 with human (87.5%), rat (98.3%), and dog (92.9%) orthologs.
  • mAsb-17 expression is exclusively detected in the mouse testis.
  • Expression begins by the fourth week after birth and continues into adulthood, with peak levels in round spermatids.

Conclusions:

  • mAsb-17 is a testis-specific gene in mice.
  • The expression profile suggests a critical role for mASB-17 in male reproductive functions, specifically in testis development and spermatogenesis.