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Updated: Aug 21, 2026

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time
Published on: October 26, 2016
Electrochromatographic studies of etched capillaries modified with a cyano pentoxy biphenyl liquid crystal
G Brent Dawson1, Maria T Matyska, Joseph J Pesek
1Department of Chemistry, University of North Carolina, Greensboro, Greensboro, NC 27403, USA.
Abstract:
A liquid crystal stationary phase for open tubular capillary electrochromatograpy (OTCEC) is fabricated by etching a fused silica tube and then bonding 4,4'-cyanopentoxy biphenyl by a silanization/hydrosilation process. The versatility of this electrophoretic capillary is demonstrated by separations of proteins, peptides, basic pharmaceuticals and the metabolites of tryptophan. Chromatographic interactions are verified by resolution of two neutral peptides. Variable temperature studies are used to understand the liquid crystal properties of the bonded moiety. EOF measurements as a function of pH and temperature further characterize this unique separation media.
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