Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ecotoxicological effects at contaminated sites.

Karl Fent1

  • 1Institute of Environmental Technology, University of Applied Sciences Basel, St. Jakob Strasse 84, CH-4132 Muttenz, Switzerland; Department of Environmental Sciences, Swiss Federal Institute of Technology (ETH), CH-8092 Zürich, Switzerland. k.fent@fhbb.ch

Toxicology
|October 7, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Suspect List-Guided High-Throughput Screening (SLIG-HTS) Identifies Environmental Substances Associated with Advanced Embryonic Developmental Timing.

Environmental science & technology·2026
Same author

Identification of toxic substances considering circadian disruption: an integrated approach combining reduced transcriptomic analysis, behavioral monitoring, and time-course gene expression profiling.

Environment international·2026
Same author

Genetic Polymorphisms in Nuclear Receptors Drive Variations in Responses to Environmental Exposures.

Environmental science & technology·2025
Same author

Endogenous hormones matters in evaluation of endocrine disruptive effects mediated by nuclear receptors.

Eco-Environment & Health·2024
Same author

AOP-Anchored Transcriptome Analysis Catalogue Accelerates the Discovery of Environmental Toxicants in Zebrafish.

Environmental science & technology·2024
Same author

Prednisolone Accelerates Embryonic Development of Zebrafish via Glucocorticoid Receptor Signaling at Low Concentrations.

Environmental science & technology·2023

Contaminated sites threaten ecosystems with long-term chronic effects. Novel in vitro methods using fish cell lines offer a faster, more sensitive approach to assess ecotoxicological risks from pollutants like polycyclic aromatic hydrocarbons (PAH).

Area of Science:

  • Environmental Science and Ecotoxicology
  • Environmental Chemistry
  • Biomarker Research

Background:

  • Contaminated sites represent significant environmental hazards, impacting terrestrial, groundwater, and aquatic ecosystems through acute and chronic ecotoxicological effects.
  • Pollutants often exhibit high toxicity, persistence, mobility, and lipophilicity, leading to bioaccumulation and complex interactions within ecosystems.
  • Bioavailability is a critical factor determining the ecotoxicological impact of contaminants, as exemplified by persistent organotins in aquatic environments.

Purpose of the Study:

  • To evaluate the ecotoxicological risks posed by contaminated sites, focusing on the limitations of traditional assessment methods.
  • To introduce and validate novel in vitro systems, specifically fish cell lines, for rapid and sensitive ecotoxicological hazard and risk assessment.
  • To develop a concept for assessing polycyclic aromatic hydrocarbon (PAH) contamination using induction equivalents applicable to both aquatic and terrestrial ecosystems.

Related Experiment Videos

Main Methods:

  • Utilized fish cell lines as a rapid and reliable in vitro system for evaluating contaminated sediments and landfill leachates.
  • Assessed acute toxicity through cytotoxicity measurements.
  • Measured the induction of cytochrome P4501A (CYP1A) as a sensitive biomarker for exposure and toxicity.

Main Results:

  • In vitro assays with fish cell lines proved effective for hazard and risk assessment of PAH-contaminated samples.
  • Cytotoxicity and CYP1A induction served as important indicators of contaminant toxicity.
  • A concept based on induction equivalents for ecotoxicological evaluation of PAH contamination was developed.

Conclusions:

  • In vitro fish cell line systems offer a promising alternative to traditional ecotoxicological tests, providing faster and more sensitive hazard assessments.
  • The developed induction equivalent concept aids in evaluating PAH contamination across different ecosystems.
  • Further research is needed to address the largely unknown long-term chronic ecotoxicological effects on soil and aquatic biota.