Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fluorescent proteins as sensors for cellular functions.

Oliver Griesbeck1

  • 1Nachwuchsgruppe Zelluläre Dynamik, Max-Planck-Institut für Neurobiologie, Am Klopferspitz 18, 82152 Martinsried, Germany. griesbeck@neuro.mpg.de

Current Opinion in Neurobiology
|October 7, 2004
PubMed
Summary

Green fluorescent protein biosensors are revolutionizing nervous system imaging. Genetically encoded sensors are advancing functional imaging from single cells to whole organisms in vivo.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Morphotype-specific calcium signaling in human microglia.

Journal of neuroinflammation·2024
Same author

Calcium flow at ER-TGN contact sites facilitates secretory cargo export.

Molecular biology of the cell·2024
Same author

Fluorescent sensors for imaging of interstitial calcium.

Nature communications·2023
Same author

Probing the interstitial calcium compartment.

The Journal of physiology·2022
Same author

CRISPR/Cas9-based directed evolution in mammalian cells.

Current opinion in structural biology·2021
Same author

Targeted In Situ Protein Diversification and Intra-organelle Validation in Mammalian Cells.

Cell chemical biology·2020

Area of Science:

  • Neuroscience
  • Biotechnology
  • Molecular Imaging

Background:

  • Green fluorescent protein (GFP)-based biosensors offer potential for revolutionizing functional imaging of the nervous system.
  • Numerous reporters for neuronal activity and biochemical signaling pathways have been developed.

Purpose of the Study:

  • To highlight the progress and challenges in translating GFP-based biosensors from laboratory settings to in vivo imaging applications.
  • To discuss the potential of genetically encoded sensors in advancing neuroscience research.

Main Methods:

  • Review of current advancements in GFP-based biosensor development.
  • Analysis of strategies for probe development and application.
  • Examination of genetically encoded sensors and their expression in model organisms.

Related Experiment Videos

Main Results:

  • A significant increase in the diversity of available GFP-based probes.
  • Challenges remain in transitioning probes from in vitro to in vivo imaging.
  • Successful functional expression of new genetically encoded sensors in model organisms.

Conclusions:

  • The field is progressing towards in vivo functional imaging of the nervous system using advanced biosensors.
  • Genetically encoded sensors represent a promising direction for future neuroscience research.
  • Further development is needed to bridge the gap between probe design and whole-organism imaging.