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Rapid quantitative detection of Listeria monocytogenes in meat products by real-time PCR
David Rodríguez-Lázaro1, Anna Jofré, Teresa Aymerich
1Institute of Food and Agricultural Technology, University of Girona, Campus Montilivi s/n, E-17071 Girona, Spain.
Abstract:
We describe a quick and simple method for the quantitative detection of Listeria monocytogenes in meat products. This method is based on filtration, Chelex-100-based DNA purification, and real-time PCR. It can detect as few as 100 CFU/g and quantify as few as 1,000 CFU/g, with excellent accuracy compared to that of the plate count method. Therefore, it is a promising alternative for the detection of L. monocytogenes in meat products.
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