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Chromosome aberration test for hydroxyapatite in sheep.

T P Kannan1, N L Nik Ahmad Shah, A Azlina

  • 1School of Dental Sciences, Universiti Sains Malaysia, Health Campus, 16150 Kubang Kerian, Kelantan, Malaysia.

The Medical Journal of Malaysia
|October 8, 2004
PubMed
Summary

Synthetic hydroxyapatite biomaterial showed no mutagenicity or cytotoxicity in sheep blood. Karyological studies confirmed its safety, with no observed chromosome aberrations after implantation.

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Area of Science:

  • Biomaterials Science
  • Toxicology
  • Sheep Hematology

Background:

  • Synthetic hydroxyapatite is a widely used biomaterial in bone regeneration.
  • Assessing the in vivo biocompatibility of biomaterials is crucial before clinical application.
  • Sheep are a relevant animal model for studying biomaterial interactions due to physiological similarities with humans.

Purpose of the Study:

  • To evaluate the mutagenicity and cytotoxicity of dense synthetic hydroxyapatite (SHA) in sheep.
  • To determine if SHA implantation induces chromosome aberrations in sheep blood cells.

Main Methods:

  • Dense SHA was implanted into the tibia of indigenous Malaysian sheep (Malin breed).
  • Blood samples were collected pre-implantation and six weeks post-implantation.

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  • Karyological studies, including mitotic index and chromosome aberration analysis, were performed on cultured blood cells.

    • Main Results:

    • Karyological analysis revealed no significant cytotoxicity in sheep blood cells post-SHA implantation.
    • No statistically significant increase in chromosome aberrations was observed compared to pre-implantation levels.
    • Mitotic indices remained within normal ranges, indicating no adverse effects on cell proliferation.

    Conclusions:

    • Dense synthetic hydroxyapatite demonstrates a lack of mutagenicity and cytotoxicity in the tested sheep model.
    • The biomaterial is considered safe for in vivo use, showing no adverse effects on sheep blood karyology.
    • These findings support the biocompatibility of dense SHA for potential orthopedic and regenerative applications.