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Universal mouse reference RNA derived from neonatal mice.

Xiao-Rui He1, Chunlian Zhang, Cam Patterson

  • 1University of North Carolina, Chapel Hill, NC 27599-7126, USA.

Biotechniques
|October 9, 2004
PubMed
Summary
This summary is machine-generated.

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Researchers developed a simple, inexpensive method to create a reproducible mouse reference RNA from whole neonatal mice. This new whole mouse reference RNA is highly representative for accurate microarray data comparisons.

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Accurate comparisons in spotted microarray experiments require reproducible, transcriptionally diverse common reference RNA.
  • Existing methods for creating reference RNA include pooling RNAs, amplifying pooled RNAs, or synthesizing complementary nucleic acids.

Purpose of the Study:

  • To develop a simple, quick, reliable, reproducible, and inexpensive method for preparing a large amount of mouse reference RNA.
  • To assess the representativeness of the newly prepared mouse reference RNA compared to commercial alternatives.

Main Methods:

  • Preparation of reference RNA from whole neonatal mice.
  • Comparison of the whole mouse reference RNA with two commercially available universal mouse reference RNAs.

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Main Results:

  • A large amount of mouse reference RNA was successfully prepared using the described approach.
  • The whole mouse reference RNA demonstrated high representativeness when compared to commercial reference RNAs.

Conclusions:

  • The developed method provides a simple, cost-effective, and reliable way to generate high-quality mouse reference RNA.
  • This whole mouse reference RNA is suitable for accurate data normalization and comparison across different spotted microarray experiments.