Related Experiment Video
Updated: Aug 21, 2026
Endurance Training Protocol and Longitudinal Performance Assays for Drosophila melanogaster
Published on: March 26, 2012
Age-related RNA decline in adult Drosophila melanogaster
Nuzha M A Tahoe1, Ali Mokhtarzadeh, James W Curtsinger
1Department of Ecology, Evolution and Behavior, University of Minnesota, 100 Ecology, 1987 Upper Buford Circle, St. Paul 55108, USA. nmtahoe@tc.umn.edu
Abstract:
We investigated the correlation between age and total RNA levels in long-lived and control lines of Drosophila melanogaster. Total RNA was extracted at 10 ages from 1-63 days posteclosion from 3 inbred lines, with replication. Three different methods of RNA quantitation gave highly correlated estimates. Total RNA declined substantially with age, exhibiting a dramatic drop in the first few days of adult life. We find no evidence for a causal relationship between adult longevity and total RNA levels, since long-lived and control lines exhibited similar patterns of age-related RNA decline. These observations suggest that the dramatic decline in total RNA that occurs early in adult life does not explain the twofold differences in life span between lines. The pattern of age-specific decline coincides with published observations on age-specific metabolic rates, and suggests that 14-day-old flies are functionally senescent.
