Related Experiment Video
Updated: Aug 21, 2026

Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 (Kir4.1)
Published on: September 26, 2015
[DNA binding activity of nuclear factor-kappa B in human astrocytoma]
Chun-Bao Guo1, Feng Chen, Zong-Wei Gao
1Laboratory of Respiratory, Pediatric Hospital, Fudan University, Shanghai, 200032, P.R.China. guochunbao@hotmail.com
Background & Objective:
Transcription factors of human cancer cells can regulate the expression of many malignancy-related gene products,relate to poor differentiation,and high metastasis of cancer cells,and also inhibit apoptosis of cancer cells. This study was designed to investigate the nuclear factor-kappa B (NF-kappaB)DNA binding activity in human astrocytoma tissue,and its significance with human astrocytoma development.
Methods:
The NF-kappaB DNA binding activities of 12 cases of normal brain tissue, and 37 cases of human astrocytoma tissue were detected by electrophoretic mobility shift assay (EMSA), and photo densitometry.
Results:
The absorbency of NF-kappaB DNA binding activity in human astrocytoma tissue was 134.2+/-24.1, higher than that in normal brain tissue (97.5+/-1.9)(P< 0.05). The NF-kappaB DNA binding activity in multiform glioblastoma tissue was highest (106.8+/-7.4), that in anaplasia Astrocytoma was 123.2+/-10.1, in astroblastoma was 139.3+/-16.8,and in astrocytoma was 160.2+/-18.6 (P< 0.05).
Conclusion:
The NF-kappaB DNA binding activity correlates with malignancy degree of human astrocytoma.
Related Concept Videos
NF-κB-dependent Signaling Pathway
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
Co-activators and Co-repressors
The Nucleolus
Regulation of Nuclear Protein Sorting
Eukaryotic Transcription Inhibitors
Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...
Cell Specific Gene Expression

