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Updated: Aug 21, 2026

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres
Published on: July 22, 2020
[Induction of HSF1 expression and sporadic colorectal cancer]
Yong-ming Fang1, Qi Dong, Hui Cen
1The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China.
Objective:
To explore the activation of the signal transduction pathways related with the carcinogenesis of sporadic colon cancers.
Methods:
A gene microarray monitoring activation of 8 signal transduction pathways (PathwayFinder GEArray) was used to screen the differentially expressed genes between colorectal cancer and normal colon tissue. The differentially expressed genes were further analyzed by RT-PCR, using RNA extracted from cancer tissue and matched normal colon mucosa of 35 patients with colorectal cancer.
Results:
The expression of hsf1, hsf27 and inos was increased in colon cancer compared with normal colon mucosa using PathwayFinder GEArray. The RT-PCR results showed that the expression of hsf1 was detected in 86% of patients(30/35)and the expression of inos detected in 63% patients(22/35).
Conclusion:
Hsf1 induces heat shock stress signaling pathway, which might play a role in the carcinogenesis of sporadic colorectal cancer.
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