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Updated: Aug 21, 2026
Use of Single Molecule Fluorescent In Situ Hybridization (SM-FISH) to Quantify and Localize mRNAs in Murine Oocytes
Published on: April 24, 2019
Fluorescence in situ hybridization analysis of human oocytes: advantages of a double-labeling procedure
Franck Pellestor1, Tal Anahory, Brigitte Andréo
1CNRS-UPR 1142, Montpellier, France. Franck.Pellestor@igh.cnrs.fr
Objective:
To improve the fluorescence in situ hybridization (FISH) chromosomal analysis of human oocytes and first polar bodies.
Design:
In situ chromosomal identification on isolated cells, with combinations of centromeric (or locus-specific) probes and whole-chromosome painting probes for chromosomes 9, 13, 16, 18, 21, and X.
Setting:
Montpellier University Hospital.
Patient(S):
Women participating in an IVF program.
Intervention(S):
Fifty-four in vitro unfertilized oocytes were fixed on slides, and simple or double FISH labeling procedures were performed on preparations.
Main Outcome Measure(S):
Simultaneous in situ visualization of specific domains and chromosome arms of each targeted chromosome.
Result(S):
Eight chromosomal abnormalities were identified, including two hyperhaploidies, three cases of extra single chromatid, and three cases of balanced separation of sister chromatids. Also, the double-labeling procedure allowed the avoidance of five interpretation errors, owing to additional artefactual signals.
Conclusion(S):
By ensuring precise identification of both chromosomes and single chromatids, the FISH double-labeling procedure limits the risk of erroneous interpretation and allows a more accurate cytogenetic analysis of human oocytes.
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Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...