Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Crossing Over01:34

Crossing Over

147.9K
Unlike mitosis, meiosis aims for genetic diversity in its creation of haploid gametes. Dividing germ cells first begin this process in prophase I, where each chromosome—replicated in S phase—is now composed of two sister chromatids (identical copies) joined centrally.
The homologous pairs of sister chromosomes—one from the maternal and one from the paternal genome—then begin to align alongside each other lengthwise, matching corresponding DNA positions in a process...
147.9K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

5.9K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.9K
Polytene Chromosomes02:04

Polytene Chromosomes

10.2K
Polytene chromosomes are giant interphase chromosomes with several DNA strands placed side by side. They were discovered in the year 1881 by Balbiani in salivary glands, intestine, muscles, malpighian tubules, and hypoderm of larvae Chironomus plumosus. Hence, these are also called "Salivary gland chromosomes." These are found in insects of the order Diptera and Collembola; in certain organs of mammals; and synergids, antipodes of flowering plants. Polytene chromosomes are also...
10.2K
Meiosis I01:49

Meiosis I

194.4K
Meiosis is a carefully orchestrated set of cell divisions, the goal of which—in humans—is to produce haploid sperm or eggs, each containing half the number of chromosomes present in somatic cells elsewhere in the body. Meiosis I is the first such division, and involves several key steps, among them: condensation of replicated chromosomes in diploid cells; the pairing of homologous chromosomes and their exchange of information; and finally, the separation of homologous chromosomes by...
194.4K
Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

5.7K
The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
5.7K
Cohesins02:20

Cohesins

4.7K
Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of...
4.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparative induced pluripotent stem cell models of young, aged, and progeroid, as a resource to study human aging trajectories.

Stem cell research·2026
Same author

Antenatal discovery of mosaic trisomy 14 in an early-onset malformative syndrome.

Molecular cytogenetics·2026
Same author

Systematic analysis of snRNA genes reveals frequent RNU2-2 variants in dominant and recessive developmental and epileptic encephalopathies.

Nature genetics·2026
Same author

Hypertrophic Cardiomyopathy as a Key Feature of MRAS-Related Noonan Syndrome: New Case and Comprehensive Literature Review.

Prenatal diagnosis·2026
Same author

DNA methylation episignature for Smith-Magenis and Potocki-Lupski syndromes: a mirror perspective.

European journal of human genetics : EJHG·2025
Same author

Systematic analysis of snRNA genes reveals frequent <i>RNU2-2</i> variants in dominant and recessive developmental and epileptic encephalopathies.

medRxiv : the preprint server for health sciences·2025
Same journal

Isolation of Mesenchymal Stem Cell-Derived Extracellular Vesicles.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Modeling Melanoma Immune Surveillance by CAR-T Cells in Human Skin Organoids.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Stepwise Optimization of a Matrigel-Based In Vitro Angiogenesis Assay for Reproducible and Quantifiable 2D-Tube Formation Using HUVECs.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Quantifying Mechanical Properties of Fresh Ovarian Tissue with Optical Brillouin Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

3D Chromatin Architecture During Early Development: New Methods and New Findings.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Metabolic Plasticity in Embryogenesis Throughout the Lens of NAD<sup></sup>.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Sep 9, 2025

Author Spotlight: Unraveling the Dynamics of Eukaryotic DNA Replication Through Single-Molecule Visualization
07:37

Author Spotlight: Unraveling the Dynamics of Eukaryotic DNA Replication Through Single-Molecule Visualization

Published on: September 27, 2024

1.7K

Chromothripsis.

Franck Pellestor1,2, Benjamin Ganne3,4, Vincent Gatinois3,4

  • 1Unit of Chromosomal Genetics and Research Platform Chromostem, Department of Molecular Genetics and Cytogenomics, Site Unique de Biologie (SUB), Montpellier CHU, Montpellier Cedex 5, France. f-pellestor@chu-montpellier.fr.

Methods in Molecular Biology (Clifton, N.J.)
|August 30, 2025
PubMed
Summary
This summary is machine-generated.

Chromothripsis, a type of complex genomic rearrangement, involves massive chromosomal fragmentation and reassembly. This phenomenon, observed across species, drives rapid genome evolution and may explain speciation.

Keywords:
Abortive apoptosisCancerChromatin bridgeChromosome missegregationChromothripsisEvolutionGenomic instabilityMicronucleus

More Related Videos

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
17:14

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Published on: December 10, 2012

14.1K
Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II
10:39

Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II

Published on: February 26, 2018

15.7K

Related Experiment Videos

Last Updated: Sep 9, 2025

Author Spotlight: Unraveling the Dynamics of Eukaryotic DNA Replication Through Single-Molecule Visualization
07:37

Author Spotlight: Unraveling the Dynamics of Eukaryotic DNA Replication Through Single-Molecule Visualization

Published on: September 27, 2024

1.7K
Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
17:14

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Published on: December 10, 2012

14.1K
Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II
10:39

Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II

Published on: February 26, 2018

15.7K

Area of Science:

  • Genomics
  • Molecular Biology
  • Evolutionary Biology

Background:

  • Chromothripsis, a complex genomic rearrangement, was identified in 2011 and is now classified under chromoanagenesis.
  • This phenomenon occurs in various conditions, including cancers, congenital diseases, and in healthy individuals across diverse species.

Purpose of the Study:

  • To characterize the molecular features distinguishing chromothripsis from other complex rearrangements.
  • To elucidate the cellular mechanisms driving chromothripsis and its role in genome reorganization.

Main Methods:

  • Analysis of molecular characteristics to define chromothripsis.
  • Review of experimental models detailing the cascade of cellular events leading to chromothripsis.

Main Results:

  • Chromothripsis is characterized by specific molecular signatures, differentiating it from other complex chromosomal rearrangements.
  • Three primary mechanisms initiate chromothripsis: micronuclei formation, chromatin bridges from telomeric fusions, and abortive apoptosis.
  • These processes involve fragmentation, repair, and transmission of damaged chromosomal material.

Conclusions:

  • Chromothripsis is a significant discovery from high-resolution genome analysis, revealing a mechanism for rapid genome modification.
  • It plays a crucial role in germline and early developmental genome alterations, supporting macroevolution and speciation concepts.