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Updated: Aug 21, 2026

Defining Substrate Specificities for Lipase and Phospholipase Candidates
Published on: November 23, 2016
Coupled-enzyme system for the determination of lipase activity
Ines Ben Rejeb1, Jeannette Ben Hamida, Mohamed Gargouri
1Department of Biological & Chemical Engineering, National Institute of Applied Science and Technology (INSAT), Tunis, Tunisia.
Abstract:
A new method for lipase activity determination is described, in which liberation of fatty acids by lipase-catalyzed hydrolysis is coupled to lipoxygenation. The second reaction forms hydroperoxy-fatty acids containing a conjugated double bond that are detected at 234 nm. The method is sensitive, cheap and easy to use when compared to a titration method.
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