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Recycling of the dense-core vesicle membrane protein phogrin in Min6 beta-cells

Yen P Vo1, John C Hutton, Joseph K Angleson

  • 1Department of Biological Sciences, University of Denver, Denver, CO 80208, USA.

Biochemical and Biophysical Research Communications
|October 16, 2004
PubMed

Phogrin (IA2-beta) is an integral membrane protein of dense-core vesicles in neuroendocrine cells. We have examined the recycling of endogenous phogrin following exocytosis in insulin secreting Min6 beta-cells by monitoring stimulus dependent-uptake of antibodies directed against the lumenal domain of the protein. While low levels of internalized phogrin accumulated in LAMP1-positive lysosomes, more than 35% of internalized phogrin recycled back to an insulin-positive compartment and could return to the cell surface during a second exocytic stimulation. The recycling phogrin transited a syntaxin 6-positive compartment but did not appear to go through the TGN38-positive trans Golgi network. The results suggest a model in which secretory membrane components can recycle from the endosomal system to immature secretory granules without interaction with the major portion of the TGN.

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