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From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
Published on: January 13, 2017
Rapid cloning and expression of a fungal polyketide synthase gene involved in squalestatin biosynthesis
Russell J Cox1, Frank Glod, Deirdre Hurley
1School of Chemistry, University of Bristol, Cantock's Close, Bristol, UK BS8 1TS. r.j.cox@bris.ac.uk.
Chemical Communications (Cambridge, England)
|October 19, 2004
Abstract:
PCR primers designed to selectively amplify the unique C-methyltransferase domain of fungal polyketide synthases were used to selectively clone a polyketide synthase gene involved in the biosynthesis of the squalene synthase inhibitor squalestatin S1 , heterologous expression of which led to the biosynthesis of the squalestatin side-chain.
