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Updated: Aug 21, 2026

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
Published on: August 14, 2019
[Two new flavonoids from the flower of Castanea mollissima Blume]
Si Wang1, Wen-zhao Tang, Xing-bao Ding
1Institute of Materia Medica, Shandong Academy of Medical Science, Jinan 250062, China.
Aim:
To study the bioactive constituents from the flower of Castanea mollissima Blume.
Methods:
Compounds were isolated and purified by column chromatography on silica gel and polyamide. Structures were determined by various spectroscopic data,including UV, IR, 1H and 13CNMR, EIMS and FABMS, 1H-13C-COSY and HMBC.
Results:
Two compounds were isolated from the ethyl acetate fraction of 95% ethanol extract and the structures were elucidated as kaempferol-3-O-[6"-(E)-p-coumaroyl]-alpha-D-mannopyranoside (1) and kaempferol-3-O-[6", 4"-di-(E)-p-coumaroyl]-alpha-D-mannopyranoside (2).
Conclusion:
Compounds 1 and 2 are new compounds, named castanoside A and B respectirely.
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