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Related Experiment Videos

Multidrug transporter activity in lymphocytes.

James I Elliott1, Selina Raguz, Christopher F Higgins

  • 1Membrane Transport Biology Group, MRC Clinical Sciences Centre, Faculty of Medicine, Imperial College, Hammersmith Hospital Campus, Du Cane Rd, London W12 0NN, UK. james.elliot@csc.mrc.ac.uk

British Journal of Pharmacology
|October 20, 2004
PubMed
Summary

Murine B and T lymphocytes have active multidrug transporters, contrary to prior beliefs. This finding reveals a previously unknown drug import activity and impacts immune disorder treatments.

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Area of Science:

  • Immunology
  • Pharmacology
  • Cell Biology

Background:

  • Multidrug transporters (MDTs) are crucial in hematopoietic cells, affecting xenobiotic efflux and cell migration.
  • Conflicting reports exist on MDT distribution in lymphocytes due to limited specific antibodies.
  • Previous studies suggested murine B cells lack transporter activity.

Purpose of the Study:

  • To clarify the distribution and activity of MDTs in murine lymphocytes.
  • To investigate potential drug import activities in these cells.
  • To understand the role of MDTs in immune cell function and therapeutic barriers.

Main Methods:

  • Analysis of parental and knockout mice models.
  • Functional assays to detect transporter activity.

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  • Investigation of drug-specific import mechanisms.

    • Main Results:

    • Contrary to previous findings, murine B and T lymphocytes possess at least three active MDTs.
    • A novel, drug-specific import activity was identified in these lymphocytes.
    • P-glycoprotein (P-gp) drug specificity was found to be cell type-dependent.

    Conclusions:

    • Murine lymphocytes express active multidrug transporters and exhibit drug import functions.
    • Developmentally regulated MDTs can create barriers to treating immune disorders.
    • These findings necessitate a re-evaluation of MDT roles in lymphocyte function and drug delivery.