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Updated: Aug 21, 2026

Voltage-Dependent Potassium Current Recording on H9c2 Cardiomyocytes via the Whole-Cell Patch-Clamp Technique
Published on: November 11, 2022
Effects of amiloride on potassium and calcium currents in guinea pig ventricular myocytes
Lan Cheng1, Fang Wang, Hong-Yi Zhou
1Department of Pharmacology, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China. chenglan1@hotmail.com
Aim:
To elucidate the possible mechanisms underlying antiarrhythmia of the non-selective Na+/H+ exchanger inhibitor--amiloride.
Methods:
Single ventricular cells were isolated using a double-enzyme method. Effects of amiloride on voltage-dependent potassium and calcium currents in isolated guinea pig ventricular myocyte were recorded by using whole-cell patch clamp techniques.
Results:
Exposure to amiloride (10 -100 micromol x L(-1)), the L-type and T-type calcium currents were depressed. Amiloride resulted in a concentration-dependent inhibition of peak (Ca,L), But amiloride did not change the shape of their I - V curves. It only decreased the amplitudes of the currents of the two types. When myocytes were incubated with 100 micromol x L(-1) amiloride, I(Kr) was slightly depressed and I(Ks) did not change. Amiloride (1 - 100 micromol x L(-10) depressed I(K1) in a concentration-dependent manner.
Conclusion:
Amiloride depressed potassium and calcium currents, which may give support to its uses in some diseases of the cardiovascular system.
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