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Structural study on cultured isolated fetal rat pancreatic islets
Faisal M Al-Mashat1, Abdulrahman M Sibiany, Mostafa M El-Naggar
1Department of Surgery, College of Medicine, King Abdul-Aziz University, Jeddah, Kingdom of Saudi Arabia. falmashat@yahoo.com
Saudi Medical Journal
|October 21, 2004
Summary
Cultured fetal rat pancreatic islets showed increasing diameter and purity over five days, despite a decreasing number. These findings offer insights into islet culture for research.
Area of Science:
- Endocrinology
- Developmental Biology
- Histology
Background:
- Fetal rat pancreatic islets are crucial for studying diabetes and islet transplantation.
- Understanding the morphometric and structural changes during islet culture is essential for optimizing protocols.
Purpose of the Study:
- To conduct a detailed morphometric and structural analysis of isolated fetal rat pancreatic islets during a five-day culture period.
Main Methods:
- Islets were isolated from 22-day-old fetal rats using collagenase digestion.
- Islets were cultured for 1-5 days, with measurements of count, diameter, and purity.
- Histological examination involved fixation, dehydration, embedding, and H&E staining.
Main Results:
- Islet purity and diameter significantly increased with culture duration.
- Islet number progressively decreased over the culture period.
- Histological analysis confirmed the structural integrity of cultured islets.
Conclusions:
- The study provides extensive morphometric and structural data on cultured fetal rat pancreatic islets.
- Results indicate that islet diameter and purity improve with culture time, while islet count declines.