Changes in the luminescence between dried and wet bacillus spores

Fluorescence has been suggested as a method with which to detect and identify bacterial spores. To better understand the nature of the fluorescence signal, we observed the intrinsic steady-state fluorescence and phosphorescence spectra of Bacillus globigii (BG) in both dried and aqueous forms. In vitro, dried, and suspension forms of BG were measured at room temperature in 300-600-nm excitation wavelengths. Also, the phosphorescence of dry BG spores was measured at room temperature at 300-600-nm excitation wavelengths. The wet BG spores exhibited a strong maximum in their fluorescence spectrum, with the peak excitation wavelength near 300 nm and emission wavelength near 400 nm. When the BG was dried, this peak shifted to an approximately 450-nm excitation maximum and an 500-nm emission maximum. The difference between the wet and the dry spore fluorescence spectra cannot be explained by the phosphorescence of the dry spores. Other changes must take place when the spores are wet to account for the large changes observed in the spectrum.