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Updated: Aug 21, 2026

Murine Orchiectomy and Ovariectomy to Reduce Sex Hormone Production
Published on: November 17, 2023
Ovariectomy enhances renal cortical expression and function of cyclooxygenase-2
Yuko Tada1, Atsuhiro Ichihara, Yukako Koura
1Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan.
Background:
Cyclooxygenase-2 (COX-2) inhibitors are used as analgesics in postmenopausal women, who develop edema and require a salt-restricted diet. This study was performed to determine the renal expression of COX-2 and on COX-2-dependent regulation of renal blood flow (RBF) in ovariectomized rats.
Methods:
Sprague-Dawley rats were divided into 4 groups: sham-operated rats fed a normal-salt diet (Sh+NS) or a low-salt diet (Sh+LS), and bilaterally ovariectomized rats fed a normal-salt diet (Ox+NS) or a low-salt diet (Ox+LS) (N= 6 in each group). Estrogen replacement therapy was performed on other ovariectomized rats. A renal clearance study was performed in anesthetized animals.
Results:
Ovariectomy increased renal cortical COX-2 expression independently of dietary salt intake (Sh+NS
Conclusion:
Estrogen-dependent COX-2 expression plays an important role in the RBF regulation in female rats.
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