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Related Experiment Videos

Does aging affect liver microtubules?

L Taylor1, A L Jones, D L Schmucker

  • 1Cell Biology and Aging Section, Department of Veterans Affairs Medical Center, San Franscisco, California 94121.

Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)
|April 1, 1992
PubMed
Summary
This summary is machine-generated.

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Aging impairs microtubule function, reducing vesicle transport in cells. This age-related decline in microtubules and associated proteins may serve as a biomarker for aging.

Area of Science:

  • Cell Biology
  • Aging Research
  • Cytoskeleton Dynamics

Background:

  • Microtubules are crucial for cellular processes like endocytosis and vesicle transport.
  • Age-related changes in the cytoskeleton, particularly microtubules, can lead to cell dysfunction.
  • Vesicle translocation is significantly reduced in aged rats compared to younger ones.

Purpose of the Study:

  • To investigate age-related changes in microtubule concentration and function.
  • To determine the impact of aging on tubulin and microtubule-associated proteins (MAPs).
  • To explore the potential of microtubule alterations as aging biomarkers.

Main Methods:

  • Comparative analysis of microtubule profiles in young and old rats using electron microscopy.
  • Quantification of polymerized and total tubulin concentrations at different ages.

Related Experiment Videos

  • Assessment of free fractions of microtubule-associated proteins (MAPs).

    • Main Results:

    • A significant decline (greater than 70%) in polymerized tubulin by 12 months of age.
    • Total tubulin content remained stable until 24 months, then declined by 50%.
    • Increased free fractions of MAP1 and heat-stable MAPS in aged rats, indicating reduced association with polymerized tubulin.

    Conclusions:

    • Aging is associated with significant reductions in polymerized tubulin and potential alterations in microtubule structure.
    • Increased free MAP fractions suggest impaired microtubule assembly or stability with age.
    • These age-associated microtubule perturbations may serve as reliable biomarkers of the aging process.