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Published on: July 27, 2018
[Detection and sequence analysis of Sapporo-like virus in Guangzhou]
Yi Liu1, Ying-chun Dai, Jian-dong Li
1Department of Epidemiology, Southern Medical University, Guangzhou 510515, China. ly811@fimmu.com
Objective:
To verify the presence of Sapporo-like virus (SLV) in children with diarrhea in Guangzhou.
Methods:
Stool specimens were collected from children with diarrhea treated in the Department of Pediatrics of Nanfang Hospital in autumn and winter, 2003, and semi-nested reverse transcriptase-polymerase chain reaction (RT-PCR) was employed for SLV detection, the positive product of which was sequenced and analyzed in comparison with the SLV sequences documented in GenBank.
Results:
Only one positive specimen (CH03354) was found out of the 169 stool specimens collected. Sequence analysis of the capsid gene of this strain showed that it belonged to SLV GI-1 cluster.
Conclusion:
SLV exists in Guangzhou and different regions of China may have different genotypes of this virus.