Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Epidermal cell analysis by RT-PCR.

Tammy-Claire Troy1, Robert Man-Kit Cheung, Kursad Turksen

  • 1Ottawa Health Research Institute, Ottawa, Ontario, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|October 27, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tissue barriers and their impact.

Tissue barriers·2024
Same author

Building barriers.

Tissue barriers·2017
Same author

Preface. Human Embryonic Stem Cell Protocols.

Methods in molecular biology (Clifton, N.J.)·2015
Same author

Preface. Stem Cell Renewal and Cell-Cell Communication.

Methods in molecular biology (Clifton, N.J.)·2015
Same author

Occlusive lung arterial lesions in endothelial-targeted, fas-induced apoptosis transgenic mice.

American journal of respiratory cell and molecular biology·2015
Same author

All about claudins.

Tissue barriers·2014
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

This study details a reliable reverse transcription polymerase chain reaction method for analyzing epidermal gene expression. This technique is crucial for novel genes where antibody availability limits other methods.

Area of Science:

  • Molecular Biology
  • Dermatology
  • Genetics

Background:

  • Epidermal gene expression analysis is vital in dermatology.
  • Immunolocalization is often limited by the lack of antibodies for novel genes.
  • Semiquantitative analysis is needed for gene expression studies.

Purpose of the Study:

  • To present a reliable reverse transcription polymerase chain reaction (RT-PCR) method.
  • To enable semiquantitative analysis of epidermal gene expression.
  • To provide a method applicable to both in vivo and in vitro studies.

Main Methods:

  • Reverse transcription polymerase chain reaction (RT-PCR) protocol.
  • Semiquantitative gene expression analysis.
  • Application in both in vivo and in vitro epidermal models.

Related Experiment Videos

Main Results:

  • A reliable RT-PCR method for epidermal gene expression was established.
  • The method allows for semiquantitative analysis.
  • Successful application in both in vivo and in vitro settings.

Conclusions:

  • RT-PCR is a valuable tool for epidermal gene expression analysis.
  • This method overcomes limitations of immunolocalization for novel genes.
  • The described protocol is robust for diverse experimental conditions.