Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Keratinocyte transient transfections.

Anthony M Flores1, Brian J Aneskievich

  • 1Department of Pharmaceutical Sciences, School of Pharmacy, University of Connecticut, Storrs, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 27, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inflammation and epidermal barrier integrity, decline, and restoration: It's time to rouse the "guardians at the gate".

Tissue barriers·2026
Same author

The intrinsically disordered protein TNIP1 forms puncta in cells and undergoes liquid-liquid phase separation in vitro.

International journal of biological macromolecules·2026
Same author

Conformational Analyses of the AHD1-UBAN Region of TNIP1 Highlight Key Amino Acids for Interaction with Ubiquitin.

Biomolecules·2025
Same author

Protocol Development for CRISPR/Cas9 Knockout of the Anti-inflammatory Protein TNIP1 in HaCaT Keratinocytes.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Critical Analysis of Cytoplasmic Progression of Inflammatory Signaling Suggests Potential Pharmacologic Targets for Wound Healing and Fibrotic Disorders.

Biomedicines·2025
Same author

Investigating Protein-Protein Interactions of Autophagy-Involved TNIP1.

Methods in molecular biology (Clifton, N.J.)·2024
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

This guide offers proven, cost-effective transient transfection techniques for keratinocytes and other cell lines. It emphasizes repeatable methods adaptable for various experimental scales and conditions.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biotechnology

Background:

  • Transient transfection is crucial for gene expression studies.
  • Optimizing transfection protocols for specific cell types like keratinocytes is essential.
  • Existing methods may lack cost-effectiveness or adaptability.

Purpose of the Study:

  • To provide a clear guide to reliable transient transfection techniques for keratinocytes.
  • To detail methods using accessible and inexpensive resources.
  • To ensure techniques are repeatable and scalable for diverse experimental needs.

Main Methods:

  • Standard precipitation protocol for general transfection.
  • Poly-L-ornithine protocol for calcium-sensitive applications.
  • Inclusion of essential basic cell culture techniques for optimal results.

Related Experiment Videos

Main Results:

  • The described techniques are effective for primary keratinocytes and continuous cell lines.
  • Methods are adaptable for COS-7 and CV-1 cell lines.
  • Detailed notes enhance user guidance for novice and experienced scientists.

Conclusions:

  • The chapter presents practical, resource-efficient transient transfection protocols.
  • These methods support a range of experimental designs and cell types.
  • Success is contingent on factors like promoter strength and cell culture quality.