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Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display
Colin M Leaf1, Pearl Qi2, Richard W Roberts3,4,5
1Department of Chemistry, University of Southern California, Los Angeles, CA, 90089, USA.
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mRNA display is a powerful in vitro selection technique that enables the discovery of peptide and protein ligands from libraries exceeding 10 trillion unique sequences. The technique allows for exquisite control over binding stringency and specificity, and it is highly amenable to high-throughput ligand profiling and integration with machine learning approaches. Here, we describe general methods for performing mRNA display selections.
